Ubaashana - in the (save) push button callback, you will save the data to file, possibly using imwrite. For example,
function pushbutton1_Callback(hObject, eventdata, handles)
% get the image data to save (from an axes or the handles structure)
imgToSave = ...;
% save to file
filename = ...;
imwrite(imgToSave, filename);
I'm assuming that you can easily get the watermarked image from the handles structure and that the filename is known (or are you asking the user for the filename?).
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