Shil - in the OpeningFcn of your GUI, just set the Data property of the uitable to be a 2x2 cell array. Something like the following should work
function myGUI_OpeningFcn(hObject, eventdata, handles, varargin)
handles.output = hObject;
guidata(hObject, handles);
set(handles.uitable1,'Data',cell(2,2));
The above was tested with R2014a.
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