Hello everybody,
I've been trying to segment this image for some time, and chiefly I need to identify the nuclei as signified by dark, roughly circular objects. Original image (Photobucket)
My current train of thought uses a Canny edge detect, and then a circular Hough transform from there: Edge detected image. However, as you can see in my edge detection, there are a lot of edges which clearly don't correspond to nuclei, and I'm wondering if it would improve things significantly if I could discard the edges which are too long or too short to be a nucleus. To that end, is it possible to find the length of each 'chain' which is an edge, and discard them?
That's my main question, but if anybody has any suggestions that might help the problem as a whole, then that would be fantastic too!
Many thanks, Callum
Best Answer