MATLAB: Producing Graphical map of Image intensity via dual-ROI, utilizing baseline ROI

image intensityimage processingImage Processing Toolboxintensity mappingroi

Hello, Before I present my question, I'd like to make it clear that I am very new in the Matlab field, and so there is a lot of concepts and commands that I am unfamiliar with. That said, I am very eager to learn what specifically I need to do, and have already looked in the online resources to try to answer my question before I've came to the actual community.
Presented below is essentially what I'm attempting to make:
These series of images are a linear progression of image intensity as a function of temperature, and were provided to us by a collaborator whom analyzed our results. From what I understand, they were able to take a baseline image in grayscale such as the one provided below
And as you can (hopefully) tell, there are two circles present in the image, which can be used as a baseline, and then via subtracting the difference between the baseline and all images hereafter.
That is the procedure I am hoping to obtain, and receive a result such as the first image I posted, however if it is easier to obtain just a numerical value for the sphere, rather than an imaged map such as the one posted, that can be sufficient too.
I hope my request for help doesn't seem like I'm asking for somebody to do it for me, as I'm eagerly wanting to learn these commands and techniques; however I feel that this is a rather daunting task in regards to my inexperience.
Please let me know if there's any other questions I can answer, and I'll be happy to! Thank you very much!

Best Answer

First of all, run my image segmentation tutorial: http://www.mathworks.com/matlabcentral/fileexchange/?term=authorid%3A31862
Next, stitch all your images together and apply a colormap.
tallImage = [image1; image2; image3; image4];
% Then apply the jet colormap
colormap(jet(256));
colorbar;
Let us know of further questions.
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