MATLAB: Please Help me How can Extract the Blood Vessel Segmentation

Image Processing ToolboxMATLAB and Simulink Student Suitesegmentation

Hi…….I have Extract the blood vessels using fundus images but still the images was not extracted properly Preprossing i have used to Anisotropic Diffusion Filter ,Gamma correction and CHLAE and the Post- Processing method using Vessels Extraction using Adaptive Thershloding but the blood vessels are not extracted …..any other methods for using extraction and segmentation process