Please see image above. It is a merge of red and blue channels but I do separate the channels to count. I tried using the blue_count and blue_final (because this is the image where I have eliminated unwanted parts for count) instead of blue_countfilled to find the intensity as you suggested, like this:
MeanIntensityValue = blue_count(blue_countfilled); but it gave me the following error: Subscript indices must either be real positive integers or logicals.
I also get an error when I tried this code:
CC = blue_countfilled; stats = regionprops (CC,blue_count,'MinIntensity','MaxIntensity','MeanIntensity', 'PixelValues', 'Area'); T(end).stats = stats; error message: Error using regionprops>getPropsFromInput (line 1290) PROPERTY must be a string. Error in regionprops>ParseInputs (line 1244) reqStats = getPropsFromInput(startIdxForProp, ... Error in regionprops (line 205) [I,requestedStats,officialStats] = ParseInputs(imageSize, argOffset, varargin{:});
I am using Matlab to count cells in a tissue section. I use the following code to count the cells:
-blue_count = bwconncomp(blue_final); -blue_num = blue_count.NumObjects;After counting, I am interested in measuring the intensity of pixels per cell (per area of the cell). First, I used imfill : -blue_countfilled = imfill (blue_final, 'holes'); -figure, imshow (blue_countfilled) -T(end). imfill = imfill(blue_final, 'holes');The imfill in structure T gives an output of 1040x1392 double, inside this are all zerosThen I tried a few things to try to get intensity value average for all the cells, first I used the following code: -MeanIntensityValue = mean2(blue_countfilled); -T(end).PixelIntensity = MeanIntensityValue;This output gives a value of 0.0145 which I do not trust. Then I tried the following code:CC = blue_countfilled;
This gives an output of 1 each for MinIntensity, MaxIntensity, and MeanIntensity. It gives an output of 21041×1 for PixelValues, and an area value different from what it gave initially in pixels.
I am not sure what I am doing wrong but I need to analyze intensity of stain per cell for all cells in the tissue section. PLEASE HELP.
Best Answer