MATLAB: I want to count all those object that contain black dots

Question

i want to count all those object that contain black dots?is there any method to remove all those objects that empty? here is my image. image Analyst

Answer 1

First you need to do color segmentation to find all the light purple things. That's pretty easy - see my File Exchange or use the Color Thresholder App from the Apps tab on the MATLAB tool ribbon.

Then you need to split them apart because some round cells are overlapping. For that, see this: http://blogs.mathworks.com/steve/2013/11/19/watershed-transform-question-from-tech-support/.

Then you need to label with bwlabel() and call regionprops with a binary image that is split apart but still has the black dots in it. Ask for the Euler number. Count the number of results that have a Euler number less than 1.