MATLAB: I have an skeleton image i want to find the mid point in between the branch point and end point

digital image processingImage Processing Toolboxpathpattern recognition

help me to find mid point in between two points or nodes

You should try bwdistgeodesic(). See Steve's blog.

Yes, cast it to double, if it's not already, then assign it

grayImage = double(grayImage);
grayImage(row, column) = 2.4598;
% If you want to show it, use the [] option in imshow():
imshow(grayImage, []);

I'd think taking the average of the x and y values for each endpoint would do it:

xMiddle = (x1+x2)/2;
yMiddle = (y1+y2)/2;

or am I overlooking something?