Valarie - your line of code
just opens or displays the figure corresponding to the GUI but does not perform any of the initializations that would occur when launching the GUI correctly. Replace this callback with just
function pushbutton16_Callback(hObject, eventdata, handles)
help01;
to launch the GUI when pressing this pushbutton 16. Note also, that if you want to show the image when the help GUI launches, you must put that command in the OpeningFcn of your GUI (not the pushbutton 5 unless you intend the image to load when the user presses this button).
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