MATLAB: How to load a .mat file with a push button, calculate the data using the own functions and plot a figure in a GUI made in GUIDE.

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Hi everyone,
I am very new at this with making GUIs. I have a .mat file (consisting of 4 separate variables) that I am uploading using a push button in a GUI and using the code:
function load_file_pushbutton_Callback(hObject, eventdata, handles)
% hObject    handle to load_file_pushbutton (see GCBO)
% eventdata  reserved - to be defined in a future version of MATLAB
% handles    structure with handles and user data (see GUIDATA)
filename = uigetfile('*.mat'); 
handles.data = load(filename);
I now want to change the data in the .mat file with a few functions I have written before as .m files (which I of course can copy into the GUI .m file) and plot a figure using a different pushbutton. Can anyone please help me?
Thanks in advance!

Best Answer

Andreas - yes, if your mat file consists of the variables p, t, x, and y, then if your first pushbutton callback does
 function load_file_pushbutton_Callback(hObject, eventdata, handles)
 filename = uigetfile('*.mat'); 
 handles.data = load(filename);
 guidata(hObject,handles);
then you will be able to access these variables in any other callback through (for example)
 handles.data.x
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