MATLAB: How to find major and minor axis of each human cells

Question

Hi All,

I want to find the major and minor axis of each human cell in order to find their eccentricity. So I tried to find the maximum and minimum values of X axis and Y axis of each cell. But I do not know how can I find corresponding coordinates of these maxX and maxY. (file data is a wield arraged struct, I'll attach the image of that chart below)

%eccentricity might able to work
for n = 1:length(data)
    A = data(n).coordinates(:,:,1);
    B = data(n).coordinates(:,:,2);
    X2(n) = max(A); %needs to find corresponding coordinates----bug
    X1(n) = min(A);
    Y2(n) = max(B);
    Y1(n) = min(B);
    mal(n) = sqrt((X2-X1).^2+(Y2-Y1).^2);
    nal(n) = sqrt((x2-x1).^2+(y2-y1).^2);
    ecce(n) = nal/mal;
end

Also, I found in this way, the length of major and minor axis mearsured are not really accurate. So does anyone has a faster way to find major and minor axis of human cells?

Thanks a lot for help!

Answer 1

As you have data in hand, you can try fitting a ellipse to the data and get major, minor axes. There are variety of functions to fit ellipse in fileexchange. Pick the one which suits you.

https://www.mathworks.com/matlabcentral/fileexchange/15125-fitellipse-m

https://www.mathworks.com/matlabcentral/fileexchange/3215-fit_ellipse

https://www.mathworks.com/matlabcentral/fileexchange/67313-cell-segmentation-seg-self-method

https://www.mathworks.com/matlabcentral/fileexchange?q=fit+ellipse