MATLAB: How to enhance the gradient of the contour of the object in the image

contour detectionImage Processing Toolboximage segmentation

My problem is to detect the contour of the cell and its nucleus. to detect the contour of the cell I did my job perfectly.but i can't proceed in detecting the nucleus of the cell. 
my code is.
A=imread('D:\sou project\original\2.bmp');
s=strel('disk',1,0);%Structuring element
I3 = imadjust(A);
figure, imshow(I3);

D=im2bw(I3);%binary Image F=imerode(D,s);%Erode the image by structuring element figure,imshow(A);title('Original Image'); figure,imshow(D);title('Binary Image'); figure,imshow(D-F);title('Boundary extracted Image');

i have attached the cell image below…kindly help me

Best Answer

Most likely, you don't need to do that. You're basically saying "I need algorithm A to detect and measure B" but you didn't tell us what B is . You just said you need algorithm A and I don't think that is likely to be the algorithm you need.

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MATLAB: How can i use Mean vector difference enhancer to highlight the gradient of the object contour and to suppress the gradient of noise contour

Like your duplicate question, I don't think you need to use that algorithm. You can do the code I gave you in your duplicate question http://www.mathworks.com/matlabcentral/answers/151808#comment_233588

MATLAB: How to find regions of any image using active contour

Hello Komal,

Please make your code readable. And you forgot to attach your image as well.

For detecting regions in an image, you can use this method as well:

 clc;
close all;
grayImage = imread('random.png'); 
[rows, columns, numberOfColorBands] = size(grayImage);
if numberOfColorBands > 1
  grayImage = grayImage(:, :, 2);
end
subplot(2, 1, 1);
imshow(grayImage, []);
title('Original Grayscale Image');
binaryImage = grayImage > 128;
binaryImage = imclearborder(binaryImage);
binaryImage = bwareaopen(binaryImage, 1000);
subplot(2, 1, 2);
imshow(binaryImage, []);
title('Binary Image');

It works pretty well and is able to detect all type of regions.

I have attached my image as well. Use that for proper understanding.

Result:

Best Answer

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MATLAB: How can i use Mean vector difference enhancer to highlight the gradient of the object contour and to suppress the gradient of noise contour

MATLAB: How to find regions of any image using active contour

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