It appears that the GUI was made using App Designer / Guide and relies on dependent files being present in the current directory. Note that a compiled application behaves more consistently if it avoids accessing or changing the current directory. A more reliable way is to base all file locations on a known root i.e. ctfroot for deployed applications. For example:
load('c:/Work/MATLAB/data.mat')
[pathstr,name]=fileparts(which('data.mat'));
load(name);
To further narrow down the exact point of error, on a Windows Machine before compiling, Under Additional runtime settings -> uncheck "Do not display the Windows Command Shell' and check 'Create log file'.
The Python error is also likely connected to the path issues of the standalone application.
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