Hello, how can I convert this grayimage back to an rgb with green fluorescence?
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MATLAB: Grayimage back to green
gray imagegray to rgbimage processing
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No. There is no way to obtain the original gray level image from a binary image that has been created by thresholding that gray scale image. If you want the gray scale image later, be sure to save your gray scale image variable.
Here's the full demo for you:
clc; % Clear the command window.
close all; % Close all figures (except those of imtool.)
clear; % Erase all existing variables. Or clearvars if you want.
workspace; % Make sure the workspace panel is showing.
format short g;format compact;fontSize = 25;%===============================================================================
% Get the name of the image the user wants to use.
baseFileName = 'o4s1_11.bmp';% Get the full filename, with path prepended.
folder = pwdfullFileName = fullfile(folder, baseFileName);%===============================================================================% Read in a first image.
grayImage1 = imread(fullFileName);% Get the dimensions of the image.
% numberOfColorChannels should be = 1 for a gray scale image, and 3 for an RGB color image.
[rows, columns, numberOfColorChannels] = size(grayImage1)if numberOfColorChannels > 1 % It's not really gray scale like we expected - it's color.
% Use weighted sum of ALL channels to create a gray scale image.
% grayImage = rgb2gray(grayImage);
% ALTERNATE METHOD: Convert it to gray scale by taking only the green channel,
% which in a typical snapshot will be the least noisy channel.
grayImage1 = grayImage1(:, :, 2); % Take green channel.
end% Display the image.
subplot(2, 2, 1);imshow(grayImage1, []);axis on;axis image;caption = sprintf('Image1');title(caption, 'FontSize', fontSize, 'Interpreter', 'None');drawnow;hp = impixelinfo();% Set up figure properties:
% Enlarge figure to full screen.
set(gcf, 'Units', 'Normalized', 'OuterPosition', [0, 0.04, 1, .96]);% Get rid of tool bar and pulldown menus that are along top of figure.
% set(gcf, 'Toolbar', 'none', 'Menu', 'none');
% Give a name to the title bar.
set(gcf, 'Name', 'Demo by ImageAnalyst', 'NumberTitle', 'Off')drawnow;%===============================================================================% Read in a second image.
fullFileName = fullfile(pwd, 'blankv5.bmp');grayImage2 = imread(fullFileName);% Get the dimensions of the image.% numberOfColorChannels should be = 1 for a gray scale image, and 3 for an RGB color image.[rows, columns, numberOfColorChannels] = size(grayImage2)if numberOfColorChannels > 1 % It's not really gray scale like we expected - it's color. % Use weighted sum of ALL channels to create a gray scale image.% grayImage = rgb2gray(grayImage); % ALTERNATE METHOD: Convert it to gray scale by taking only the green channel, % which in a typical snapshot will be the least noisy channel. grayImage2 = grayImage2(:, :, 2); % Take green channel.end% Display the image.subplot(2, 2, 2);imshow(grayImage2, []);axis on;axis image;caption = sprintf('Image2');title(caption, 'FontSize', fontSize, 'Interpreter', 'None');drawnow;hp = impixelinfo();% Display the histogram of the image so we can see what threshold to use.
subplot(2, 2, 3);histogram(grayImage2);grid on;% Binarize the image.
threshold = 128;binaryImage = grayImage1 < threshold; % Determine number from histogram.
% Get rid of surround that is touching the border.
binaryImage = imclearborder(binaryImage);% Display the image.subplot(2, 2, 3);imshow(binaryImage, []);axis on;axis image;caption = sprintf('Binary Image');title(caption, 'FontSize', fontSize, 'Interpreter', 'None');drawnow;hp = impixelinfo();% Use this binary image to transfer the stuff in the second image.
grayImage2(binaryImage) = grayImage1(binaryImage);% Display the image.subplot(2, 2, 4);imshow(grayImage2, []);axis on;axis image;caption = sprintf('Final Image');title(caption, 'FontSize', fontSize, 'Interpreter', 'None');drawnow;
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