Dear all,
I have a 360X300 matrix that represent fluorescent intensities along the line. Each cell in a column (1:360), has a certain intensity value that changes in time (rows 1:300). The fluorescent signal is slightly moving through time, so at time 0 is in column e.g., 2, than in time point 2, it is in column 5, etc. I would like to draw the line on top of the figure tracing the position of the signal at each time frame, so get the information about the position of the signal at each time frame, i.e. time1 – position 5 time2 – position 7 time3 – position 9, etc.
The signal is noisy so it has to be done manually, this way, no peak finding or similar. I used imfreehand but is gives me information about XY coordinates and not the exact [row column].
Thanks in advance, jakub
Best Answer