You were so close:
figure
scatter([0 10 20 30 40],[3 5 3 2 9]);
hold on
plot([0 40],[15 15],'-', 'Color','r')
If you really wanted to use line(), you can do so, but you need to set its colour after it's been created:
figure
scatter([0 10 20 30 40],[3 5 3 2 9]);
hold on
h = line([0 40],[15 15])
set(h,'Color','r')
Best Answer