MATLAB: Am I unable to view the difference between these two images

imageimage analysisimage processingmatricesmatrixmatrix manipulation

I used two different programs to align a series of frames in a tiff file to later analyze. Out of curiosity, I'm wondering if each program yielded the same set of aligned images. Say program 1 spit out file1 and program 2, file2. How can I check to see that the difference is a zero matrix if the following specifications for file1 and file2 are as follows:
FileSize: 196788
                       Format: 'tif'
                FormatVersion: []
                        Width: 256
                       Height: 256
                     BitDepth: 24
                    ColorType: 'truecolor'
              FormatSignature: [73 73 42 0]
                    ByteOrder: 'little-endian'
               NewSubFileType: 0
                BitsPerSample: [8 8 8]
                  Compression: 'Uncompressed'
    PhotometricInterpretation: 'RGB'
                 StripOffsets: 180
              SamplesPerPixel: 3
                 RowsPerStrip: 256
              StripByteCounts: 196608
                  XResolution: 1
                  YResolution: 1
               ResolutionUnit: 'None'
                     Colormap: []
          PlanarConfiguration: 'Chunky'
                    TileWidth: []
                   TileLength: []
                  TileOffsets: []
               TileByteCounts: []
                  Orientation: 1
                    FillOrder: 1
             GrayResponseUnit: 0.0100
               MaxSampleValue: [255 255 255]
               MinSampleValue: [0 0 0]
                 Thresholding: 1
                       Offset: 8
    FileSize: 162046
                         Format: 'tif'
                  FormatVersion: []
                          Width: 256
                         Height: 256
                       BitDepth: 16
                      ColorType: 'grayscale'
                FormatSignature: [73 73 42 0]
                      ByteOrder: 'little-endian'
                 NewSubFileType: 0
                  BitsPerSample: 16
                    Compression: 'LZW'
      PhotometricInterpretation: 'BlackIsZero'
                   StripOffsets: 8
                SamplesPerPixel: 1
                   RowsPerStrip: 256
                StripByteCounts: 161900
                    XResolution: []
                    YResolution: []
                 ResolutionUnit: 'Inch'
                       Colormap: []
            PlanarConfiguration: 'Chunky'
                      TileWidth: []
                     TileLength: []
                    TileOffsets: []
                 TileByteCounts: []
                    Orientation: 1
                      FillOrder: 1
               GrayResponseUnit: 0.0100
                 MaxSampleValue: 65535
                 MinSampleValue: 0
                   Thresholding: 1
                         Offset: 161908
                   SampleFormat: 'Unsigned integer'
Matlab is complaining that Matrix dimensions don't agree. I've done a similar analysis on different frames from the same tiff file and haven't run into issues, so what's wrong?
file1 = '/Users/...';
file2 = '/Users/...';
A = double(imread(file1,1));
B = double(imread(file2,1));
C = A - B

Best Answer

 Matlab is complaining that Matrix dimensions don't agree.
Please post a copy of the error message instead of paraphrasing it. The message is clear: A and B have different sizes. Use the debugger to check this:
size(A)
size(B)
As Walter has pointed out already, one image is a true-color image (3 dimensions) and the other one a gray scale image (2 dimensions). Then you cannot subtract them.
The debugger is very useful to solve such questions:
dbstop if error
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