MATLAB: About brightness

brightness

[EDIT: 20110523 17:15 CDT – reformat. clarify – WDR]
Hello everyone, I have a trouble with the brightness in a pic that I take:
I'm new in Matlab anyone can help me please? I need to remove the brightness in the edges, then to binarize just to see the white points, the picture is of bacterial colonies. Thanks!
PS: sorry for my bad English.

Best Answer

I suggest im2bw perhaps in combination with graythresh
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