MATLAB: Separate two type of object in the image

image processingImage Processing Toolboximage segmentation

How to separate the two types of blobs in the input image?

Best Answer

Do you mean like this:
clc; % Clear the command window.
close all; % Close all figures (except those of imtool.)
clear; % Erase all existing variables. Or clearvars if you want.
workspace; % Make sure the workspace panel is showing.
format long g;
format compact;
fontSize = 22;
%--------------------------------------------------------------------------------------------------------

% READ IN IMAGE
folder = pwd;
baseFileName = 'Q4_4.png';
% Get the full filename, with path prepended.
fullFileName = fullfile(folder, baseFileName);
% Check if file exists.
if ~exist(fullFileName, 'file')
% The file doesn't exist -- didn't find it there in that folder.
% Check the entire search path (other folders) for the file by stripping off the folder.
fullFileNameOnSearchPath = baseFileName; % No path this time.
if ~exist(fullFileNameOnSearchPath, 'file')
% Still didn't find it. Alert user.
errorMessage = sprintf('Error: %s does not exist in the search path folders.', fullFileName);
uiwait(warndlg(errorMessage));
return;
end
end
grayImage = imread(fullFileName);
% Get the dimensions of the image.
% numberOfColorChannels should be = 1 for a gray scale image, and 3 for an RGB color image.
[rows, columns, numberOfColorChannels] = size(grayImage)
if numberOfColorChannels > 1
% It's not really gray scale like we expected - it's color.
% Use weighted sum of ALL channels to create a gray scale image.
grayImage = rgb2gray(grayImage);
% ALTERNATE METHOD: Convert it to gray scale by taking only the green channel,
% which in a typical snapshot will be the least noisy channel.
% grayImage = grayImage(:, :, 2); % Take green channel.
end
% Display the image.
subplot(2, 3, 1);
imshow(grayImage);
axis('on', 'image');
title('Original Grayscale Image', 'FontSize', fontSize, 'Interpreter', 'None');
impixelinfo;
hFig = gcf;
hFig.WindowState = 'maximized'; % May not work in earlier versions of MATLAB.
drawnow;
%--------------------------------------------------------------------------------------------------------
% Binarize the image.
binaryImage = ~imbinarize(grayImage);
% Get rid of particles less than 200 in area.
binaryImage = bwareaopen(binaryImage, 200);
% Eliminate partial blobs - those touching the edge of the image.
binaryImage = imclearborder(binaryImage);
subplot(2, 3, 2);
imshow(binaryImage, []);
axis('on', 'image');
title('Mask Image', 'FontSize', fontSize, 'Interpreter', 'None');
impixelinfo;
drawnow;
% Find blobs
props = regionprops(binaryImage, 'Centroid', 'Area');
xy = vertcat(props.Centroid)
allAreas = [props.Area];
subplot(2, 3, 3);
histogram(allAreas, 10);
grid on;
title('Histogram of Blob Areas', 'FontSize', fontSize, 'Interpreter', 'None');
xlabel('Area', 'FontSize', fontSize, 'Interpreter', 'None');
ylabel('Count', 'FontSize', fontSize, 'Interpreter', 'None');
% Get blobs smaller than 2500
[labeledImage, numBlobs] = bwlabel(binaryImage);
smallIndexes = find(allAreas < 2500);
largeIndexes = find(allAreas >= 2500);
% Extract the small blobs with ismember()
smallBlobs = ismember(labeledImage, smallIndexes);
subplot(2, 3, 4);
imshow(smallBlobs);
title('Small Blobs', 'FontSize', fontSize, 'Interpreter', 'None');
% Extract the large blobs with ismember()
largeBlobs = ismember(labeledImage, largeIndexes);
subplot(2, 3, 5);
imshow(largeBlobs);
title('Large Blobs', 'FontSize', fontSize, 'Interpreter', 'None');
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