I have a video in which a cell moves along a channel…however, the background i.e. the channel is near the brightness of that of my cell, so when I convert the video into images of its frames, and turn them into grayscale images, I cannot isolate the cell from its background very clearly, though I can faintly see it move against the background.
I tried frame subtraction..after subtracting the first frame that does not contain the cell from the remaining, I still get a lot of background noise. *Thus, when I try locating the cell by trying to identify the maximum brightness spot, *I get the locations of different other points with the greater brightness than the cell.
Can you suggest what I can do?