I have a problem visualizing a stack/multipage tiff file with both ImageJ (Bio-Format plug-in) and MATLAB. In the MATLAB file there seems to be more noise or higher background values compared to the ImageJ image. This is not in the visualization, but the ratio between signal (circles) and background differs between the two images. However, I cannot find if there is something wrong with my MATLAB script in visualizing the images. As I thought it can simply be done with imread (see code below).
Image = '0000127_01_800.TIF';info = imfinfo('0000127_01_800.TIF');num_images = numel(info);for k = 1:num_images A = imread(Image,k,'Info', info); B{k} = Aend
Does someone has experience with both ImageJ and MATLAB who can maybe help me to understand this difference?
Thanks!
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